2019-nCoV (ORF1ab/E/N Gene) Nucleic Acid Detection Kit (Fluorescence PCR)
For in vitro qualitative detection of SARS-CoV-2 (also known as 2019-nCoV) ORF1ab, E, and N gene in oropharyngeal swabs and sputum specimens of suspected SARS-CoV-2-infected pneumonia cases, suspected clustered infection cases, and others who need SARS-CoV-2 infection diagnosis or differential diagnosis.
Description
1. Principle
This product is designed as a multiplex real-time reverse-transcription PCR system, containing specific primers and fluorescent probes targeting ORF1ab, E and N gene of SARS-CoV-2. Virus nucleic acid is detected by monitoring fluorescence intensity in real-time. Internal control is added to monitor the presence of PCR inhibitor within specimen, thus effectively prevent false.negative results.
2. Product characteristics：
（1）Triple detection to prevent missed detection
（2）Add internal standard and monitor the whole process
（3）Simulate viruses to ensure quality
（4）Optimized processes, efficient and safe
3. Operation steps:
① Reagent preparatio
② Take out and equilibrate all reagent components of this kit and a nucleic acid extraction or purification kit at ambient temperature, vortex briefly.
③ Prepare qRT-PCR Mix
④ Sample preparation
⑤ PCR amplification
4. Result judgement：
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