1.Principle This product is designed as a multiplex real-time reverse-transcription PCR system, containing specific primers and fluorescent probes targeting ORF1ab, E and N gene of SARS-CoV-2. Virus nucleic acid is detected by monitoring fluorescence intensity in real-time. Internal control is added to monitor the presence of PCR inhibitor within specimen, thus effectively prevent false.negative results. 2.Product characteristics: (1)Triple detection to prevent missed detection (2)Add internal standard and monitor the whole process (3)Simulate viruses to ensure quality (4)Optimized processes, efficient and safe 3.Operation steps: ① Reagent preparation ② Take out and equilibrate all reagent components of this kit and a nucleic acid extraction or purification kit at ambient temperature, vortex briefly. ③ Prepare qRT-PCR Mix ④ Sample preparation ⑤ PCR amplification

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