Description
Products Description


2×YT Agar specification is 250g per bottle. For the cultivation of Escherichia coli.
Instructions for use:
1.Suspend 46.0 g in 1 L of purified water
2.Heat with frequent agitation and boil to completely dissolve the powder
3.Dispense and autoclave at 121 ℃ for 20 minutes
Ingredients :
| Formula (1L) | Amount(g) |
| Tryptone | 16.0 |
| Yeast extract | 10.0 |
| Sodium Chloride | 5.0 |
| Agar | 15.0 |
| Final pH (25℃ ) | 7.0±0.2 |

| Cat.# | Description | Package |
| YTA-250 | 2×YT Agar Medium | 250g/Bottle, 10 Bottles/Case |
Frequently Asked Questions
Q1: What is the specification of 2×YT Agar?
A: Each bottle contains 250 g of 2×YT Agar powder, suitable for routine E. coli cultivation.
Q2: How do I prepare 1 L of 2×YT Agar medium?
A: Suspend 46.0 g of powder in 1 L of purified water.
Heat with stirring and bring to a boil to fully dissolve the components.
Dispense into containers and sterilize by autoclaving at 121 °C for 20 minutes.
Q3: What is the final pH of the medium?
A: The pH is adjusted to 7.0 ± 0.2 at 25 °C before sterilization.
Q4: Which applications is 2×YT Agar best suited for?
A: Designed for high‑yield E. coli growth, plasmid rescue, and routine cloning workflows.
Biologix is specialized in the R & D and production of disposable medical detection reagents & supplies, laboratory supplies & instruments, and complete biobanking solutions. It has the standard class 100,000 clean room which are compliant with GMP standards and number of professional R & D testing laboratories which are all ISO 9001, CE, and FDA registered, and follow the strict CDC guidelines for purity and efficiency. Biologix is committed to the design and manufacture of high-quality products, enriching product lines, and strengthening the service of complete solutions.

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