EDTA Buffer
EDTA Buffer is a pH 8.0 metal ion chelating buffer that serves as a highly efficient hydrophilic chelating agent. It specifically binds to divalent metal ions such as Mg²+, Ca²+, Mn²+, and Fe²+, forming stable inactivated complexes. This effectively inhibits the activity of metal ion-dependent enzymes (e.g., nucleases and proteases).
Description
Products Description
Feature
| Cat # |
Product Name
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Formula
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Package
|
|
EB-500
|
EDTA Buffer
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/ |
500mL/ Bottle, 10 Bottles/Case
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Frequently Asked Questions
Q1: What is EDTA Buffer used for?
A1: EDTA Buffer (pH 8.0) chelates divalent metal ions to inhibit metal‑dependent enzymes, making it ideal for applications such as nuclease and protease inhibition, DNA/RNA preservation, and sample stabilization.
Q2: Which metal ions does it bind?
A2: It strongly complexes Mg²⁺, Ca²⁺, Mn²⁺, and Fe²⁺, forming stable, inactive chelates.
Q3: How does EDTA Buffer inhibit enzyme activity?
A3: By removing essential metal cofactors from solution, it prevents metal‑dependent enzymes (e.g., DNases, RNases, proteases) from functioning.
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