Electrophoresis Buffer
High quality: The buffers are filtered by 0.4µm to ensure the highest quality Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.
Description
Products Description
Features
Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation
time while reducing the likelihood of buffer concentration errors.
Selection Guide
● Nucleic acid electrophoresis
When high resolution is not necessary, both TAE and TBE buffers are suitable. For higher resolution requirements, using a lower concentration gel is advantageous for improving the resolution of large molecular weight nucleic acids (>13kb), and TAE isrecommended. Conversely, higher concentration gels are beneficial for enhancing the resolution of small molecular weight nucleic acids, and TBE is preferable in this case.
● Protein Electrophoresis
Neutral pH buffers for the Bis-Tris gel system help to improve sample integrity and gel stability, resulting in clearer, sharper bands and higher resolution for low protein abundance or downstream applications requiring high protein integrity. MOPs buffer is recommended for separating proteins of 15 - 260 kDa. As for the proteins of 3.5 - 160 kDa, MES buffer is recommended. Electrophoresis on Tris-Glycine gel system, TGS (TG+0.1% SDS) buffer is recommended.
● Protein blotting
Transfusion buffers are used in WB experiments to transfer proteins from gels to membranes, and then to semi-quantitatively detect a protein in complex samples based on antigen-antibody specific binding.
| Cat # |
Product Name
|
Formula
|
Package
|
|
TAE-500
|
TAE pH8.0 buffer(1×)
|
40 mM Tris-acetate, 1 mM EDTA
|
500mL/ Bottle, 10 Bottles/Case
|
|
TGS-500
|
TGS buffer(1×)
|
25 mM Tris, 192 mM Glycine, 0.1% SDS
|
500mL/ Bottle, 10 Bottles/Case
|
|
TG-500
|
TG buffer(1×)
|
25 mM Tris, 192 mM Glycine
|
500mL/ Bottle, 10 Bottles/Case
|
|
TG-510
|
TG buffer(10×)
|
0.25 M Tris, 1.92 M Glycine
|
500mL/ Bottle, 10 Bottles/Case
|
|
WTB-510
|
WTB transfer buffer(10×)
|
0.25 M Tris, 1.92 M Glycine
|
500mL/ Bottle, 10 Bottles/Case
|
|
MOPS-500
|
Tris-MOPS buffer(1×)
|
50 mM Tris, 50 mM MOPS, 1 mM EDTA, 0.1% SDS
|
500mL/ Bottle, 10 Bottles/Case
|
|
MES-500
|
Tris-MES buffer(1×)
|
50 mM Tris, 50mM MES, 1 mM EDTA, 0.1% SDS
|
500mL/ Bottle, 10 Bottles/Case
|
|
SDS-500
|
SDS Buffer
|
10% SDS
|
500mL/ Bottle, 10 Bottles/Case
|
Frequently Asked Questions
Q1: What types of electrophoresis buffers does Biologix offer?
A1: Biologix provides pre‑made buffers for agarose and polyacrylamide gel electrophoresis, suitable for both nucleic acid and protein separation.
Q2: What are the advantages of using Biologix pre‑made buffers?
A2: They are made from purity‑tested reagents and filtered through 0.4 μm membranes to ensure consistent quality. Pre‑made format reduces preparation time and avoids concentration errors.
Q3: How do I choose between TAE and TBE for DNA/RNA electrophoresis?
A3:For high molecular weight nucleic acids (>13 kb): Use low‑concentration gels with TAE buffer. For small molecular weight fragments: Use high‑concentration gels with TBE buffer.
Biologix is specialized in the R & D and production of disposable medical detection reagents & supplies, laboratory supplies & instruments, and complete biobanking solutions. It has the standard class 100,000 clean room which are compliant with GMP standards and number of professional R & D testing laboratories which are all ISO 9001, CE, and FDA registered, and follow the strict CDC guidelines for purity and efficiency. Biologix is committed to the design and manufacture of high-quality products, enriching product lines, and strengthening the service of complete solutions.
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