NuclEase-Free Water, 100ml/500ml
Multiple distillation and sterilization treatments. It is Pyrogen-free, DNase/RNase-free, protease-free, bacteria-free, and DEPC-free. It can be used to prepare various high-concentration solutions in the laboratory. PH4.0-6.0.
Description
Product Overview
Features
1.Triple‑distilled and sterile‑filtered for maximum purity
2.Free of pyrogens, nucleases (DNase/RNase), proteases, bacteria, and DEPC
3.Ideal for making concentrated laboratory reagents; pH range 4.0 – 6.0
DESCRIPTION
Nuclease‑Free Water is produced by repeated distillation and sterilization of purified water, guaranteeing the absence of pyrogens, nucleases, proteases, bacteria, and DEPC. Its ultra‑clean profile makes it suitable for biochemistry, molecular and cell biology, physiology, pathology, and other applications. Especially recommended for RNA/DNA work and any DEPC‑sensitive protocols, it also serves as a base for high‑purity solution preparation.
Storage & Shelf Life: Store at 2-30℃ . Shelf life of 1 year.
Applications
RNA Purification: Ideal for DEPC‑sensitive reverse transcription and qPCR sample prep
HPLC Solvent Base: Provides a clean baseline with minimal background noise
Custom Cell Culture Buffers: Easily formulate buffers in the pH 4.0–6.0 range
Enzyme Assays: Perfect for preparing high‑concentration SDS or PMSF solutions
Product Nature
| Appearance | Colorless Clear Liquid |
|---|---|
| Dnase/Rnase | Dnase/Rnase-free |
| Proteinase | None |
| Sterile | Sterile |
| Electrical Conductivity | ≤10.0 μS/cm(25℃) |
| Cat# | Description | Volume | Weight(kg) | |
|---|---|---|---|---|
| NFW-100 | NuclEase-Free Water, 100mL/Bottle, 20 Bottles/Case | 296*250*126mm,0.0093m³ | 2 | |
| NFW-500 |
|
420*180*195mm,0.0147m³ | 5.1 |
Frequently Asked Questions
Q1: Can I autoclave Nuclease‑Free Water before use?
A1: No. It is supplied sterile and ready for immediate use; additional autoclaving may introduce contaminants and alter its purity.
Q2: How should opened bottles be stored and used?
A2: Reseal tightly after each use and keep at 2 – 30 °C. For best results, use within 30 days to maintain sterility.
Q3: Is this water suitable for all molecular biology applications?
A3: Yes. Its pyrogen‑, nuclease‑, protease‑, bacteria‑, and DEPC‑free profile makes it ideal for RNA/DNA workflows, enzyme reactions, and preparation of high‑purity solutions.
Q4: What pH range does the water maintain?
A4: The water naturally falls within a pH range of 4.0 – 6.0, suitable for most laboratory buffer preparations.
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