SB Broth

 

SB Broth (Dry Powder) is a high-performance, nutrient-rich culture medium designed for high-yield molecular biology applications. With elevated levels of tryptone (32g/L) and yeast extract (20g/L) - far exceeding standard broths - it supports robust bacterial growth, making it the preferred choice for plasmid extraction and recombinant protein production.

 

Key Features & Benefits

Superior Nutrient Density: Contains 3x more tryptone and 2x more yeast extract than standard LB broth, ensuring cells reach higher densities and produce more target biomolecules (plasmids/proteins) - critical for research efficiency.

Cost-Effective Dry Format: 250g powder makes 4.4L of medium (when prepared as 1L batches), reducing shipping costs vs. liquid media and minimizing waste with easy storage.

Flexible Preparation: Scalable for small (50mL) to large (10L) cultures; simply adjust powder-to-water ratio. Autoclaving step ensures sterility without compromising nutrient quality.

Stable Long-Term Storage: Unopened bottles remain viable for 12+ months in cool, dry conditions (no refrigeration needed), ideal for labs with variable usage rates.

 

Instructions for use:

1.Weigh 57.0 g of this product

2.Dissolve it by heating in 1000 mL of distilled water

3.Dispense it into aliquots

4.Sterilize by autoclaving at 121℃ for 15 minutes before use

 

Ingredients :

Formula(1L)	Amount(g)
Tryptone	32
Yeast Paste	20
Sodium Chloride	5

 

 

 

Ordering Information

Cat.#	Product Name	Description	Qty
SB-250	SB broth	SB broth,bottled powder	250g/ Bottle, 10 Bottles/Case

 

Quality Control
Each batch of SB Broth undergoes strict quality testing:

Sterility: Verified for absence of bacterial/fungal contaminants before packaging.

Performance: Tested with E. coli cultures to ensure growth kinetics and plasmid yield meet standards.

Consistency: pH and nutrient content are calibrated to ensure batch-to-batch uniformity.

 

FAQ

1. What is this product?

This product is a dry powder SB broth medium, primarily used for plasmid and protein extraction. It contains a high amount of tryptone and yeast extract, with major nutrients far exceeding the requirements for achieving cell growth saturation.

2. What is the product specification?

The product comes in 250g per bottle.

3. How to use this product?

Follow these steps:

Weigh 57.0g of the product.

Dissolve it by heating in 1000mL of distilled water.

Dispense into aliquots.

Sterilize by autoclaving at 121℃ for 15 minutes before use.

4. Are there any storage or handling tips?

Storage: Keep the unopened bottle in a cool, dry place (below 25℃) away from direct sunlight. Once opened, seal tightly and use within 3 months to maintain nutrient activity.

Handling: Use clean, dry utensils when weighing to prevent contamination. Avoid inhaling the powder-wear gloves and a mask if necessary.

 

 

 

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