Attention Matters When Slide Smear

(1) the slides must be cleaned.

(2) the slides should be flat.

(3) the coating should be uniform.

Apply the liquid drops to the right of the slide and apply dissecting knife edge or toothpick.

(4) thin coating.

With another slide push, along with drops of slide surface (2 slide Angle should be 30 ° to 45 °) to nudge from right to left, painted a thin layer evenly.

(5) fixation.

A fixed chemical fixer or drying method (bacteria) should be fixed.

(6) dyeing.

The bacteria use methylene blue, which is used in blood, and sometimes can be used with iodine.

The dye should cover all the surfaces.

(7) washing.

Drain or bake with water absorbent paper.

(8) seal.

Long-term preservation of Canadian gum seals.